Bot. 524 PLANT TISSUE AND CELL CULTURE (2+6): 4

Bot. 524 PLANT TISSUE AND CELL CULTURE (2+6): 4
Introduction: History of plant and cell tissue culture. Basic Techniques and procedures: Types of culture from plant cells, tissues and organs, induction of callus, differentiation and morphogenesis, regeneration of plants from tissue culture. Molecular basis of regeneration: Meristem culture, embryo culture, anther culture and haploid production. Cell culture: Isolation and culture of protoplasts, somatic hybridization and genetic engineering. Tissue culture and nitrogen fixation. The role of tissue culture in basic and applied research: Production of virus free plants, improvement of productivity and propagation of useful plants. Tissue culture as a classical technique of plant improvement.