Bot. 5214 GENETIC ENGINEERING (2+3):

Bot. 5214 GENETIC ENGINEERING (2+3): 3Introduction. Definition of the science and it’s basic problems,. Solutions to the problems: Restriction and restriction mapping of genomes. Methods of gene isolation: from genomes, mRNA, immunological methods. Gel electrophoresis: Gel electrophoresis as a preparative and a diagnostic method. Ligation: enzymes, adaptors, methodologies. Gene sequencing. Cloning Vectors: Natural and recombinant vectors, properties, selection. Gene Libraries: genomic and cDNA libraries. Gene amplification: cloning and PCR. Transformation and transgensis. Role of recombinant DNA technology in industry, agriculture and biotechnology. Objectives of transgensis of Plants: resistance to pests, pathogens, improvement of horticultural and crop plants. Production of important plant products.